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virus strains herpes simplex virus type 1  (Santa Cruz Biotechnology)


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    Santa Cruz Biotechnology virus strains herpes simplex virus type 1
    Virus Strains Herpes Simplex Virus Type 1, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 46249 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 96 stars, based on 46249 article reviews
    virus strains herpes simplex virus type 1 - by Bioz Stars, 2026-09
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    FLAG-tag:

    Article Title: Unified phase separation drives DNA-induced inflammasome activation of the AIM2-like receptors.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies HA-Tag Polyclonal Proteintech 51064-2-AP; RRID: AB_11042321 HA-Tag Monoclonal Cell Signaling Technology 3724S; RRID: AB_1549585 DYKDDDDK tag Polyclonal Proteintech 20543-1-AP; RRID: AB_11232216 FLAG Tag Monoclonal Sigma F1804; RRID: AB_262044 CoraLite488-conjugated Goat Anti-Rabbit IgG Proteintech SA00013-2; RRID: AB_2797132 Anti-Mouse IgG HRP-Linked Antibody Cell Signaling Technology 7076; RRID: AB_330924 Anti-Rabbit IgG HRP-Linked Antibody Cell Signaling Technology 7074; RRID: AB_2099233 AIM2 Polyclonal Abcam ab119791; RRID: AB_10899831 GSDMD Polyclonal Abcam ab155233; RRID:AB_2736999 β-actin Monoclonal Santa Cruz sc-47778; RRID: AB_626632 Bacterial and virus strains Herpes simplex virus type 1 (HSV-1) This paper N/A Trelief® 5α Chemically Competent Cell Tsingke Biotech TSC-C01 BL21 Star (DE3) Chemically Competent Cell Tsingke Biotech TSC-E06 Chemicals, peptides, and recombinant proteins Poly(dA:dT) InvivoGen Tlrl-patn Trusza® base Sigma-Aldrich T1503 HEPES Sigma-Aldrich H3375 Sodium Chloride Sinopharm chemical reagent 10019318 Glycerol Sigma-Aldrich G6279 50%PEG8000 solution Hampton Research HR2-535 Bovine Serum Albumin Sigma-Aldrich A1933 TEV protease This paper N/A SUMO protease This paper N/A Superose 6 Increase 10/300 GL Cytiva 29091596 Hiload 16/600 Superdex 200pg Cytiva 28989335 RPMI 1640 medium MACGENE CM10040 DMEM medium MACGENE CM10013 Fetal Bovine Serum PlantChemMed PC-00001 Penicillin-Streptomycin Liquid Solarbio P1400 PMA Sigma P8139 Ni-NTA affinity chromatography Qiagen 30210 PBS Solarbio P1020 IPTG Solarbio I8070 DTT Solarbio D8220 polybrene Beyotime C0351 ChamQ SYBR qPCR Master Mix Vazyme Q712-02 HiScript II Q RT SuperMix Vazyme R223-01 Coomassie Brilliant Blue G-250 Solarbio C8420 PVDF membranes Immobilon 0000223060 Lipofectamine 3000 Thermo Fisher Scientific L3000015 Lipofectamine 2000 Thermo Fisher Scientific 11668500 Opti-MEM Thermo Fisher Scientific 32985070 (Continued on next page) Cell Reports 45, 117065, March 24, 2026 17 .. REAGENT or RESOURCE SOURCE IDENTIFIER Critical commercial assays Human IL-1β ELISA KIT Solarbio SEKH-0002 CytoTox 96 Non-Radioactive Cytotoxicity Assay Promega G1780 12.5% PAGE Gel Rapid Preparation Kit Epizyme PG113 Monolith NT.115 Capillaries Nanotemper MO-K022 Experimental models: Cell lines HEK293T cells ATCC CRL-3216 HEK293T stably expressing ASC cells Gift from Na Dong Lab N/A HEK293T cells stably expressing Doxinducible HSV-1 VP22–mCherry This paper (generated by lentiviral transduction) N/A THP-1 cells ATCC TIB-202 THP-1-AIM2 − /− cells This paper (generated by CRISPR–Cas9) N/A Oligonucleotides

    Virus:

    Article Title: Unified phase separation drives DNA-induced inflammasome activation of the AIM2-like receptors.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies HA-Tag Polyclonal Proteintech 51064-2-AP; RRID: AB_11042321 HA-Tag Monoclonal Cell Signaling Technology 3724S; RRID: AB_1549585 DYKDDDDK tag Polyclonal Proteintech 20543-1-AP; RRID: AB_11232216 FLAG Tag Monoclonal Sigma F1804; RRID: AB_262044 CoraLite488-conjugated Goat Anti-Rabbit IgG Proteintech SA00013-2; RRID: AB_2797132 Anti-Mouse IgG HRP-Linked Antibody Cell Signaling Technology 7076; RRID: AB_330924 Anti-Rabbit IgG HRP-Linked Antibody Cell Signaling Technology 7074; RRID: AB_2099233 AIM2 Polyclonal Abcam ab119791; RRID: AB_10899831 GSDMD Polyclonal Abcam ab155233; RRID:AB_2736999 β-actin Monoclonal Santa Cruz sc-47778; RRID: AB_626632 Bacterial and virus strains Herpes simplex virus type 1 (HSV-1) This paper N/A Trelief® 5α Chemically Competent Cell Tsingke Biotech TSC-C01 BL21 Star (DE3) Chemically Competent Cell Tsingke Biotech TSC-E06 Chemicals, peptides, and recombinant proteins Poly(dA:dT) InvivoGen Tlrl-patn Trusza® base Sigma-Aldrich T1503 HEPES Sigma-Aldrich H3375 Sodium Chloride Sinopharm chemical reagent 10019318 Glycerol Sigma-Aldrich G6279 50%PEG8000 solution Hampton Research HR2-535 Bovine Serum Albumin Sigma-Aldrich A1933 TEV protease This paper N/A SUMO protease This paper N/A Superose 6 Increase 10/300 GL Cytiva 29091596 Hiload 16/600 Superdex 200pg Cytiva 28989335 RPMI 1640 medium MACGENE CM10040 DMEM medium MACGENE CM10013 Fetal Bovine Serum PlantChemMed PC-00001 Penicillin-Streptomycin Liquid Solarbio P1400 PMA Sigma P8139 Ni-NTA affinity chromatography Qiagen 30210 PBS Solarbio P1020 IPTG Solarbio I8070 DTT Solarbio D8220 polybrene Beyotime C0351 ChamQ SYBR qPCR Master Mix Vazyme Q712-02 HiScript II Q RT SuperMix Vazyme R223-01 Coomassie Brilliant Blue G-250 Solarbio C8420 PVDF membranes Immobilon 0000223060 Lipofectamine 3000 Thermo Fisher Scientific L3000015 Lipofectamine 2000 Thermo Fisher Scientific 11668500 Opti-MEM Thermo Fisher Scientific 32985070 (Continued on next page) Cell Reports 45, 117065, March 24, 2026 17 .. REAGENT or RESOURCE SOURCE IDENTIFIER Critical commercial assays Human IL-1β ELISA KIT Solarbio SEKH-0002 CytoTox 96 Non-Radioactive Cytotoxicity Assay Promega G1780 12.5% PAGE Gel Rapid Preparation Kit Epizyme PG113 Monolith NT.115 Capillaries Nanotemper MO-K022 Experimental models: Cell lines HEK293T cells ATCC CRL-3216 HEK293T stably expressing ASC cells Gift from Na Dong Lab N/A HEK293T cells stably expressing Doxinducible HSV-1 VP22–mCherry This paper (generated by lentiviral transduction) N/A THP-1 cells ATCC TIB-202 THP-1-AIM2 − /− cells This paper (generated by CRISPR–Cas9) N/A Oligonucleotides

    Recombinant:

    Article Title: Unified phase separation drives DNA-induced inflammasome activation of the AIM2-like receptors.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies HA-Tag Polyclonal Proteintech 51064-2-AP; RRID: AB_11042321 HA-Tag Monoclonal Cell Signaling Technology 3724S; RRID: AB_1549585 DYKDDDDK tag Polyclonal Proteintech 20543-1-AP; RRID: AB_11232216 FLAG Tag Monoclonal Sigma F1804; RRID: AB_262044 CoraLite488-conjugated Goat Anti-Rabbit IgG Proteintech SA00013-2; RRID: AB_2797132 Anti-Mouse IgG HRP-Linked Antibody Cell Signaling Technology 7076; RRID: AB_330924 Anti-Rabbit IgG HRP-Linked Antibody Cell Signaling Technology 7074; RRID: AB_2099233 AIM2 Polyclonal Abcam ab119791; RRID: AB_10899831 GSDMD Polyclonal Abcam ab155233; RRID:AB_2736999 β-actin Monoclonal Santa Cruz sc-47778; RRID: AB_626632 Bacterial and virus strains Herpes simplex virus type 1 (HSV-1) This paper N/A Trelief® 5α Chemically Competent Cell Tsingke Biotech TSC-C01 BL21 Star (DE3) Chemically Competent Cell Tsingke Biotech TSC-E06 Chemicals, peptides, and recombinant proteins Poly(dA:dT) InvivoGen Tlrl-patn Trusza® base Sigma-Aldrich T1503 HEPES Sigma-Aldrich H3375 Sodium Chloride Sinopharm chemical reagent 10019318 Glycerol Sigma-Aldrich G6279 50%PEG8000 solution Hampton Research HR2-535 Bovine Serum Albumin Sigma-Aldrich A1933 TEV protease This paper N/A SUMO protease This paper N/A Superose 6 Increase 10/300 GL Cytiva 29091596 Hiload 16/600 Superdex 200pg Cytiva 28989335 RPMI 1640 medium MACGENE CM10040 DMEM medium MACGENE CM10013 Fetal Bovine Serum PlantChemMed PC-00001 Penicillin-Streptomycin Liquid Solarbio P1400 PMA Sigma P8139 Ni-NTA affinity chromatography Qiagen 30210 PBS Solarbio P1020 IPTG Solarbio I8070 DTT Solarbio D8220 polybrene Beyotime C0351 ChamQ SYBR qPCR Master Mix Vazyme Q712-02 HiScript II Q RT SuperMix Vazyme R223-01 Coomassie Brilliant Blue G-250 Solarbio C8420 PVDF membranes Immobilon 0000223060 Lipofectamine 3000 Thermo Fisher Scientific L3000015 Lipofectamine 2000 Thermo Fisher Scientific 11668500 Opti-MEM Thermo Fisher Scientific 32985070 (Continued on next page) Cell Reports 45, 117065, March 24, 2026 17 .. REAGENT or RESOURCE SOURCE IDENTIFIER Critical commercial assays Human IL-1β ELISA KIT Solarbio SEKH-0002 CytoTox 96 Non-Radioactive Cytotoxicity Assay Promega G1780 12.5% PAGE Gel Rapid Preparation Kit Epizyme PG113 Monolith NT.115 Capillaries Nanotemper MO-K022 Experimental models: Cell lines HEK293T cells ATCC CRL-3216 HEK293T stably expressing ASC cells Gift from Na Dong Lab N/A HEK293T cells stably expressing Doxinducible HSV-1 VP22–mCherry This paper (generated by lentiviral transduction) N/A THP-1 cells ATCC TIB-202 THP-1-AIM2 − /− cells This paper (generated by CRISPR–Cas9) N/A Oligonucleotides

    Affinity Chromatography:

    Article Title: Unified phase separation drives DNA-induced inflammasome activation of the AIM2-like receptors.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies HA-Tag Polyclonal Proteintech 51064-2-AP; RRID: AB_11042321 HA-Tag Monoclonal Cell Signaling Technology 3724S; RRID: AB_1549585 DYKDDDDK tag Polyclonal Proteintech 20543-1-AP; RRID: AB_11232216 FLAG Tag Monoclonal Sigma F1804; RRID: AB_262044 CoraLite488-conjugated Goat Anti-Rabbit IgG Proteintech SA00013-2; RRID: AB_2797132 Anti-Mouse IgG HRP-Linked Antibody Cell Signaling Technology 7076; RRID: AB_330924 Anti-Rabbit IgG HRP-Linked Antibody Cell Signaling Technology 7074; RRID: AB_2099233 AIM2 Polyclonal Abcam ab119791; RRID: AB_10899831 GSDMD Polyclonal Abcam ab155233; RRID:AB_2736999 β-actin Monoclonal Santa Cruz sc-47778; RRID: AB_626632 Bacterial and virus strains Herpes simplex virus type 1 (HSV-1) This paper N/A Trelief® 5α Chemically Competent Cell Tsingke Biotech TSC-C01 BL21 Star (DE3) Chemically Competent Cell Tsingke Biotech TSC-E06 Chemicals, peptides, and recombinant proteins Poly(dA:dT) InvivoGen Tlrl-patn Trusza® base Sigma-Aldrich T1503 HEPES Sigma-Aldrich H3375 Sodium Chloride Sinopharm chemical reagent 10019318 Glycerol Sigma-Aldrich G6279 50%PEG8000 solution Hampton Research HR2-535 Bovine Serum Albumin Sigma-Aldrich A1933 TEV protease This paper N/A SUMO protease This paper N/A Superose 6 Increase 10/300 GL Cytiva 29091596 Hiload 16/600 Superdex 200pg Cytiva 28989335 RPMI 1640 medium MACGENE CM10040 DMEM medium MACGENE CM10013 Fetal Bovine Serum PlantChemMed PC-00001 Penicillin-Streptomycin Liquid Solarbio P1400 PMA Sigma P8139 Ni-NTA affinity chromatography Qiagen 30210 PBS Solarbio P1020 IPTG Solarbio I8070 DTT Solarbio D8220 polybrene Beyotime C0351 ChamQ SYBR qPCR Master Mix Vazyme Q712-02 HiScript II Q RT SuperMix Vazyme R223-01 Coomassie Brilliant Blue G-250 Solarbio C8420 PVDF membranes Immobilon 0000223060 Lipofectamine 3000 Thermo Fisher Scientific L3000015 Lipofectamine 2000 Thermo Fisher Scientific 11668500 Opti-MEM Thermo Fisher Scientific 32985070 (Continued on next page) Cell Reports 45, 117065, March 24, 2026 17 .. REAGENT or RESOURCE SOURCE IDENTIFIER Critical commercial assays Human IL-1β ELISA KIT Solarbio SEKH-0002 CytoTox 96 Non-Radioactive Cytotoxicity Assay Promega G1780 12.5% PAGE Gel Rapid Preparation Kit Epizyme PG113 Monolith NT.115 Capillaries Nanotemper MO-K022 Experimental models: Cell lines HEK293T cells ATCC CRL-3216 HEK293T stably expressing ASC cells Gift from Na Dong Lab N/A HEK293T cells stably expressing Doxinducible HSV-1 VP22–mCherry This paper (generated by lentiviral transduction) N/A THP-1 cells ATCC TIB-202 THP-1-AIM2 − /− cells This paper (generated by CRISPR–Cas9) N/A Oligonucleotides

    Real-time Polymerase Chain Reaction:

    Article Title: Unified phase separation drives DNA-induced inflammasome activation of the AIM2-like receptors.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies HA-Tag Polyclonal Proteintech 51064-2-AP; RRID: AB_11042321 HA-Tag Monoclonal Cell Signaling Technology 3724S; RRID: AB_1549585 DYKDDDDK tag Polyclonal Proteintech 20543-1-AP; RRID: AB_11232216 FLAG Tag Monoclonal Sigma F1804; RRID: AB_262044 CoraLite488-conjugated Goat Anti-Rabbit IgG Proteintech SA00013-2; RRID: AB_2797132 Anti-Mouse IgG HRP-Linked Antibody Cell Signaling Technology 7076; RRID: AB_330924 Anti-Rabbit IgG HRP-Linked Antibody Cell Signaling Technology 7074; RRID: AB_2099233 AIM2 Polyclonal Abcam ab119791; RRID: AB_10899831 GSDMD Polyclonal Abcam ab155233; RRID:AB_2736999 β-actin Monoclonal Santa Cruz sc-47778; RRID: AB_626632 Bacterial and virus strains Herpes simplex virus type 1 (HSV-1) This paper N/A Trelief® 5α Chemically Competent Cell Tsingke Biotech TSC-C01 BL21 Star (DE3) Chemically Competent Cell Tsingke Biotech TSC-E06 Chemicals, peptides, and recombinant proteins Poly(dA:dT) InvivoGen Tlrl-patn Trusza® base Sigma-Aldrich T1503 HEPES Sigma-Aldrich H3375 Sodium Chloride Sinopharm chemical reagent 10019318 Glycerol Sigma-Aldrich G6279 50%PEG8000 solution Hampton Research HR2-535 Bovine Serum Albumin Sigma-Aldrich A1933 TEV protease This paper N/A SUMO protease This paper N/A Superose 6 Increase 10/300 GL Cytiva 29091596 Hiload 16/600 Superdex 200pg Cytiva 28989335 RPMI 1640 medium MACGENE CM10040 DMEM medium MACGENE CM10013 Fetal Bovine Serum PlantChemMed PC-00001 Penicillin-Streptomycin Liquid Solarbio P1400 PMA Sigma P8139 Ni-NTA affinity chromatography Qiagen 30210 PBS Solarbio P1020 IPTG Solarbio I8070 DTT Solarbio D8220 polybrene Beyotime C0351 ChamQ SYBR qPCR Master Mix Vazyme Q712-02 HiScript II Q RT SuperMix Vazyme R223-01 Coomassie Brilliant Blue G-250 Solarbio C8420 PVDF membranes Immobilon 0000223060 Lipofectamine 3000 Thermo Fisher Scientific L3000015 Lipofectamine 2000 Thermo Fisher Scientific 11668500 Opti-MEM Thermo Fisher Scientific 32985070 (Continued on next page) Cell Reports 45, 117065, March 24, 2026 17 .. REAGENT or RESOURCE SOURCE IDENTIFIER Critical commercial assays Human IL-1β ELISA KIT Solarbio SEKH-0002 CytoTox 96 Non-Radioactive Cytotoxicity Assay Promega G1780 12.5% PAGE Gel Rapid Preparation Kit Epizyme PG113 Monolith NT.115 Capillaries Nanotemper MO-K022 Experimental models: Cell lines HEK293T cells ATCC CRL-3216 HEK293T stably expressing ASC cells Gift from Na Dong Lab N/A HEK293T cells stably expressing Doxinducible HSV-1 VP22–mCherry This paper (generated by lentiviral transduction) N/A THP-1 cells ATCC TIB-202 THP-1-AIM2 − /− cells This paper (generated by CRISPR–Cas9) N/A Oligonucleotides



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    Figure Lengend Snippet: Antiviral activity of CTs against enveloped viruses. Two assays are reported here. (A) Co‐treatment assay against HSV‐1 and RSV in (B); (C) virus pre‐treatment assay against HSV‐1 and RSV in (D). For HSV‐1, rhamnolipid M15RL (50 μg/mL) was used in the co‐treatment and virus pre‐treatment (Giugliano et al. ); for RSV, Hylin‐a1 (50 μM) was used in co‐treatment and virus pre‐treatment assays (Chianese et al. ). Untreated but infected cells were used as controls (ctr−). The data represent the mean ± standard deviation (SD) of three independent experiments. **** p ‐value < 0.0001; * p ‐value < 0.03; ns: not significant.

    Article Snippet: The viruses used were Herpes Simplex Virus type 1 (HSV‐1) strain SC16, Respiratory Syncytial virus (RSV) (ATCC VR‐1540, Manassas, Virginia, United States), Poliovirus Type 1 (PV‐1) strain Chat (ATCC VR‐1562, Manassas, Virginia, United States), propagated on Vero‐76 cells (Cometa et al. ).

    Techniques: Activity Assay, Virus, Infection, Standard Deviation